FastQCFastQC Report
Wed 25 May 2016
SRR1780965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356658
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238796.695209416303574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACC150.002220940370.0214634
ATTGCCT150.002220940370.0214633
TCTCCGT150.002222176270.0116420
GTGCCAA150.002225887369.9821911
GCTCAAT200.00693970452.51609826
TCCGTGC200.00694355552.5087322
CGATCAT200.00694355552.5087341
GCGCTGG200.00695126252.49400715
AAGCTTG200.00695511852.4866511
GAAACTA451.6854501E-646.6547971
CTCGTAA350.001256174839.9898261
CGTAAGG350.001256174839.9898263
CACGTCA459.202921E-538.87899846
GTAGCCG400.0024135735.0107325
TGTCCGG904.478352E-935.0107332
CGTCATT400.002420221734.991148
TTGTCCG957.590643E-933.1680631
GACACGT851.0586518E-732.95127537
CGACACG851.0586518E-732.95127536
GCCAACT652.101618E-532.30400516
Produced by FastQC (version 0.11.5)