FastQCFastQC Report
Wed 25 May 2016
SRR1780965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356658
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT128193.5941994852211363No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG28950.8117019665898425No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC13140.36842016721901655No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT11000.30841870923966375TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5380.15084478688267192No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA207.916495E-570.0082839
CTCCGTT150.002223837469.998469
CGTCCGA150.002223837469.9984646
TGCCGAC401.0644726E-861.24865345
CGTCTAC553.6379788E-1157.27146548
AGGGGGG19300.056.2242670
GAATCTC700.055.006535
AATCTCG700.055.006536
GCCGACT452.6970156E-854.4432446
CAAGCTT200.006944877652.50620738
TTGGCCG200.0069487352.49884846
GGTCCCA200.0069487352.4988481
ATCAAGG200.0069487352.4988481
CTTTATA1000.052.498842
CGAATCT707.2759576E-1250.0059134
AACGTAT707.2759576E-1249.998932
CCGACTT352.0848252E-549.998947
CGAGAAC850.049.41067528
CGTATGA800.048.12394341
AACGGGG305.8705E-446.6721869
Produced by FastQC (version 0.11.5)