FastQCFastQC Report
Wed 25 May 2016
SRR1780964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307268
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306409.97175104469063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT150.002221749970.01269532
CGCTTAA150.002221749970.01269534
TAGCCCG150.002224620469.9899145
CGGAACG200.006942230352.50952524
ACGACTA200.006942230352.50952531
AGCGTAG200.006951174752.4924310
CCCGTAC200.006951174752.492433
GCCGGAA350.001253214740.00725622
GTGCGCG501.7090212E-434.9949534
TGTGCGC500.00720734827.9959623
GTGGTCG1154.886897E-827.38735648
GGTGGTC1358.038114E-925.92640947
TGGCGCT959.379115E-625.78575513
TCGGTGG1202.1599972E-623.33376745
GTGTTAG750.001831524823.329971
CACCTTA750.001831524823.329977
CGCCGTA800.00266497321.87184753
CTGGCGC800.00266497321.87184712
GTCCTAG1003.928411E-420.9969731
GGCGCTG1506.362843E-720.99697314
Produced by FastQC (version 0.11.5)