FastQCFastQC Report
Wed 25 May 2016
SRR1780964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307268
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT176665.749378392803677No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG34451.1211710949399223No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC14760.4803624197768723No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT13180.42894151034276273RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC7320.23822851712511553No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3290.10707265318874729No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTA150.00222375969.9967411
TTCAGCG150.00222375969.996749
AAGGTCG150.00222375969.9967410
GGCGGTA150.00222375969.9967424
AGGGGGG22650.058.7264770
GATTCCG200.00694849252.4975615
GAACTCG552.2755557E-950.91501236
ATCTCGA604.9149094E-946.6720937
ACTCGTA604.9149094E-946.6720938
GATCTCG32100.045.90876436
ATCTCGT31900.045.867437
CTCGTAT32250.045.80377239
CGTATGC32400.045.8003441
TGCCGTC31750.045.74590345
TCGTATG32750.045.74577340
TATGCCG31700.045.7076543
TCTCGTA32200.045.65748238
GCCGTCT31050.045.6574846
CCGTCTT30750.045.64758347
ATGCCGT32050.045.64530644