FastQCFastQC Report
Wed 25 May 2016
SRR1780963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176243
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3761921.34496121831789No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG2020.11461448114251345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT401.3460522E-1069.975646
TGGTCGC401.3460522E-1069.975649
TCGGTGG553.6379788E-1157.2527645
ATCTCGG509.731593E-1056.01226842
TGCGGTC509.767973E-1055.98047654
TGTGCGC252.3895792E-455.9804763
GTAGATC653.6379788E-1253.8732438
CTTGAGA200.00692851652.52641729
CGGTAAG200.00692851652.52641728
CCCGCAG200.00692851652.52641738
GCGAGAC200.00693630452.51149766
TCGTTCG200.00693630452.51149723
TGCGCGC200.00693630452.51149721
CAAGGCC200.00693630452.51149741
TCGTAAC200.00693630452.51149720
CGTAACA200.00693630452.51149721
AAGTCGT200.00693630452.51149717
CGGCGTG200.006951899352.48169753
CAAGTCG200.006951899352.48169716
AGGACCC200.006951899352.48169743
Produced by FastQC (version 0.11.5)