FastQCFastQC Report
Wed 25 May 2016
SRR1780963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176243
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2276212.915122870128176No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG41982.3819385734468885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC20161.1438752177391442No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT12910.7325113621533904Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC7640.43349239402415984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3970.2252571733345438No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3530.20029164278864975No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT2940.16681513592029185No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT2710.15376497222584729No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT2280.12936684010145086No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1850.1049687079770544Illumina PCR Primer Index 9 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1830.10383391113405922No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT1810.10269911429106404No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGGT150.002221770870.043
TGCCGAC455.456968E-1269.9999945
AGGGGGG28050.062.38858870
CCGACTT352.961424E-760.047
CGACTTC352.961424E-760.048
CGTATGA850.057.6470641
GCCGACT553.6379788E-1157.2727346
GTACATG252.3854662E-455.9999961
CATTGCA252.3854662E-455.99999662
TCTTTAT1350.054.4444471
AGCCGTC452.6861017E-854.44444345
CTCGTGG200.0069423252.5000041
ATGGACC200.0069423252.5000044
TTTCCAT200.0069423252.50000453
TCGTGGA200.0069423252.5000042
AGCGATC200.0069423252.5000047
GTCCGGA200.0069423252.5000041
CGTAAGA200.0069423252.50000441
GTAAGAC200.0069423252.50000442
TGTAACG200.0069423252.50000412
Produced by FastQC (version 0.11.5)