FastQCFastQC Report
Wed 25 May 2016
SRR1780962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197684
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3072015.539952651706765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAT200.0069350352.51632325
GAAATTC200.00694197652.50303615
GGCAGAC200.00694197652.50303653
GCCATAG200.006948927452.4897542
CGATCCT200.006948927452.48975465
AAGTCTC200.006948927452.48975445
CGCTTCG350.001251332240.01243668
GCGAAGT350.00125289140.0023132
ATTCGAT350.00125289140.0023132
TGCCGAA350.00125289140.0023112
GCGGCTT350.001254451339.9921911
CCACGGG350.001254451339.9921911
GGAAACA459.171138E-538.8911416
TCCGAAC400.002410923835.01088325
TCGTTTA501.7047924E-435.00202632
CGTTTAT501.7047924E-435.00202633
ACCTTTA501.7047924E-435.00202633
GGCTTAT400.00241691934.9931681
CCCCACG400.00241691934.9931689
CCTTTAG652.0944408E-532.30956334