FastQCFastQC Report
Wed 25 May 2016
SRR1780962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197684
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT175668.885898707027378No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG34491.74470366848101No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC16950.8574290281459298No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT12560.6353574391453026RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7910.4001335464681006No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3390.17148580562918597RNA PCR Primer, Index 32 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2830.14315776694117885No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2510.12697031626231764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACA150.002220292870.0149239
GGGTCCG150.002222522669.9972156
CTAAACT150.002222522669.99721523
AGGGGGG20300.059.15054370
GATGCAA200.0069377152.51119635
GGACTTG200.006944657352.4979131
AACTTGA200.006944657352.49791351
TGGAAAT200.006944657352.49791343
GACTTAT200.006944657352.4979131
AGCGCTT200.006944657352.49791310
TCATGTA200.006944657352.49791313
GCAGGAC200.006944657352.4979138
ATACGGC200.006944657352.49791317
TAACTCG407.454619E-752.49791330
CTCTCGT506.156188E-849.01045237
ATAACTC506.168557E-848.9980529
TCTTTAT1650.048.7859421
CTTTATA1700.047.3510632
TAACGGC305.8613624E-446.67661736
ATATCGT604.8967195E-946.67661737
Produced by FastQC (version 0.11.5)