FastQCFastQC Report
Wed 25 May 2016
SRR1780961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457733
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5010010.945245372302194No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT150.002224362769.9974956
GACCTTT150.002224362769.997491
TTCCGCA200.006941358652.51532738
TCCGCAC200.006947361452.5038539
GTATCAC200.006947361452.5038526
CGTTTCT200.00695036452.49811652
TTGCGGG200.00695336852.4923813
GGACGCT200.00695336852.4923813
TGCGCGT305.879879E-446.6598975
GATGCGC350.001254524240.00293468
GTTCTAT350.001255198539.9985661
TGTAGCG350.001255198539.99856616
GCATATA350.001255198539.9985661
GCGCGTC350.001255873139.9941946
GTATACC459.195166E-538.8874931
CCCGGTC459.2010865E-538.8832448
ACGTTCT708.6797263E-735.00256739
CGTTCTC708.6797263E-735.00256740
CGCGTCC708.687148E-734.99874518
GCATAGG400.00241834334.998741
Produced by FastQC (version 0.11.5)