FastQCFastQC Report
Wed 25 May 2016
SRR1780961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457733
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT301436.585280065016068No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG53911.1777608343728767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC27850.6084333006359602No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT16120.352170370062897RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC10800.2359454092232808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5380.11753576866863433No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC5320.11622496083961611No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGT150.002223770870.0021946
AGGGGGG39800.058.04835570
TAACGTA252.3865647E-456.01398538
AATAACG252.3865647E-456.01398536
AGCGTCA252.3904337E-455.995633
TCGCGCA200.006942517652.5131136
TGCGGAA200.006948520452.50163725
CTGTCGA200.006951523552.49590310
ACGTATG352.0824102E-550.0124940
CGAATGC352.0851041E-550.0015641
CCGTCTT50000.047.8114947
CGTCTTC50950.047.74437748
GCCGTCT50350.047.68768746
TATGCCG51150.047.5576943
TCTTCTG51600.047.3412750
CTCGTAT53350.047.31265639
GTATGCC51650.047.16507342
AATCTCG53150.047.16135436
CGTATGC52850.047.1537941
TCTCGTA53300.047.0943138
Produced by FastQC (version 0.11.5)