FastQCFastQC Report
Wed 25 May 2016
SRR1780960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101986
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5142650.424568077971486No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG2660.26082011256446963No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1190.11668268193673642No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG150.002213340370.04565422
GCCGGCG252.8115264E-670.04565420
CTATGGA150.002217655870.01128437
CATAATT150.002217655870.01128441
ACGACCT150.002217655870.01128428
ATATGTG150.002221977369.976954
CGCGTCT252.8279792E-669.9769447
GTCTGGC352.955676E-759.98024410
GCGTCTG352.955676E-759.9802448
TGCCGGC252.373013E-456.03652219
GCGCGTC252.3845579E-455.9815566
TCTGGCG252.3845579E-455.98155611
CCGGCGG200.006916095552.5342421
TCAGACT200.006916095552.5342424
CAGGTAG200.006929541552.50846543
TGGATTC200.006929541552.50846528
GGATTCA200.006929541552.50846529
CCGGATG200.006943006552.4827113
AAGCGCT200.006943006552.4827147
TGTGCGC200.006943006552.482713
Produced by FastQC (version 0.11.5)