FastQCFastQC Report
Wed 25 May 2016
SRR1780960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101986
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3172331.105249740160414No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG59915.874335693134352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC23652.3189457376502656No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT15581.5276606593061792Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11571.1344694369815465No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC5270.5167375914341185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4920.48241915557037235No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT3870.37946384797913435No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT3110.30494381581785734No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTAAGATCTCGTATGCCGT2660.26082011256446963No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2570.25199537191379207No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2570.25199537191379207No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT2450.24022905104622203No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT2420.23728747082932952No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2230.21865746278901024No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT2150.21081324887729685No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT2030.19904692800972681No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2010.19708587453179846No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1970.1931637675759418No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGA1660.1627674386680525No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1550.1519816445394466No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTAAGCCGT1510.1480595375835899No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1490.14609848410566156No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCAAAGATCTCGTATGCCGT1470.1441374306277332No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAG1450.14217637714980488No Hit
CTTATACACATCTCCGAGACCACGAGACTTGCTAAGATCTCGTATGCCGT1400.137273743454984No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGAATGCCGT1380.13531268997705567No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1250.12256584237052146No Hit
CTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT1110.10883846802502305No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT1090.10687741454709469No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCG1070.10491636106916635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG150.002220373169.98974
CGGCACG150.002220373169.989716
ACTTATG207.899821E-569.989750
GAGACCG150.002220373169.98979
TCCAGCA150.002220373169.98977
AGACCGC150.002220373169.989710
TCGTCGA150.002220373169.98972
GTTTTAC150.002220373169.989715
TGGCTGC150.002220373169.989723
TCTTTAT1750.067.989991
ATAAGAT501.4551915E-1162.99072632
TCCGAGA950.062.62236413
CTTGATA453.783498E-1062.24359528
ACCACGA900.062.2130719
AGGGGGG37500.062.05753770
ATATCCG401.05555955E-861.2409910
CCGAGAC1050.059.99117714
ACGTATG308.308716E-658.35336740
GCCGTAT308.308716E-658.35336746
TGCCGTA308.308716E-658.35336745
Produced by FastQC (version 0.11.5)