FastQCFastQC Report
Wed 25 May 2016
SRR1780959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473501
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG379098.006107695654286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC200.006953838352.49176411
GTCCGAA350.001254034140.00639724
CCGAACT400.00241735735.001926
GTCCCCG501.7094827E-434.9982071
TGATACG400.002419863134.99450710
GAATCCG552.993571E-431.81318941
TCCGAAC450.00430255831.11608725
TCCGTAT450.00430478331.112813
TATGTCG450.00430478331.112838
GCCGTTG450.00430478331.112826
GATCCTA1202.2955646E-929.165171
CGAGTAT604.984495E-429.1620946
ACGTTGG500.007196815728.00743729
ACACGCA500.00720424328.0015213
TTAGCGT500.00720424328.0015227
GGTACAT500.007207958527.9985661
CCGTGCG500.00721167627.9956079
TACGGTG657.944959E-426.92738255
TACCCGC657.959681E-426.91885211
GACCGGG657.959681E-426.9188527
Produced by FastQC (version 0.11.5)