FastQCFastQC Report
Wed 25 May 2016
SRR1780959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473501
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT201594.257435570357824No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG38440.8118251070219493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC18620.39324098576349364No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT10950.2312561113915282RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6780.14318871554653528No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT5030.10622997628304902No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA150.002224400469.99757446
CGGTCTT150.002224400469.99757446
ATCGGTC150.002224400469.99757444
AGGGGGG28150.055.69966570
TACGCAC200.006947575652.5037237
CCGCACG200.00695047852.4981813
ATTGACG552.2810127E-950.912729
TTGACGG506.192931E-848.99830230
CCGTCTT31600.048.0679547
CGTCTTC31500.047.6650148
GCCGTCT31550.047.58947446
GTCTTCA3300.046.6650549
CGAATGC751.4551915E-1146.66504741
TATGCCG36500.045.92991343
CGTATGC37150.045.50312841
TGCCGTC36050.045.43531845
TCGTATG38700.045.3989440
GTATGCC37300.045.32014542
CTTTATA1700.045.3021132
CTCGTAT38450.045.05695339
Produced by FastQC (version 0.11.5)