FastQCFastQC Report
Wed 25 May 2016
SRR1780958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465712
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5030310.801310681279418No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG12930.27763939945717525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCCC150.002225383769.9895869
GGTGCAC150.002225383769.9895861
TTGGGCG207.9295154E-569.9895862
GTACTAG352.9751573E-759.9910741
TGTGCGC308.379684E-658.3246573
CGACGAG252.3887763E-456.00369622
ATAACGG200.006947641352.5034644
ATTGCGG200.006950592652.4978239
TGCGTCG200.006953544452.49218811
TGCGTAC200.006953544452.49218810
GGTCTAA352.087382E-549.992561
GTGCGCG352.087382E-549.992564
TCGGTGG3200.042.6590645
GCCGTAT2550.042.54725654
CGGTGGT3100.041.77694746
CTCGGTG3000.040.8360344
TGGTCGC3050.040.1622749
TCGCCGT2850.039.2966252
AGATCTC3150.038.8872840
ATCTCGG3200.038.2837742
Produced by FastQC (version 0.11.5)