FastQCFastQC Report
Wed 25 May 2016
SRR1780958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465712
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT309146.63800803930326No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG55221.1857113409145568No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC29780.6394509911705087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24220.520063902154121No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT14570.3128542962174048Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC9990.21451025526505654No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT5700.12239323873982204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT252.8391132E-669.9967937
GGTTCCG150.00222447769.996787
AGGGGGG38300.060.2191670
ATCGTAT252.3900985E-455.9974339
CTTTATA1900.053.418592
AGATCGA200.006947767552.5032217
GATCTCC200.006947767552.5032213
GCGCCGT200.00695071952.49758511
CGTAAGA200.00695071952.49758541
TGCCGTG200.00695071952.4975857
GCCGTAT552.2810127E-950.91221246
TCGTATT352.0861138E-549.997740
TCTTCTG48150.047.6145550
ATGCAGT1250.047.5978144
CTTGAAA51900.047.4113957
GTCTTCT48500.047.3431149
GCCGTCT48650.047.34103446
CGTATGC51500.047.29879841
CGTCTTC49150.047.28672448
TCGTATG53150.047.27910640
Produced by FastQC (version 0.11.5)