FastQCFastQC Report
Wed 25 May 2016
SRR1780957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291139
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3985913.690711309718036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTA150.002225764369.9799041
CAGTTGT453.8562575E-1062.204361
GCGGACG200.006940577652.51198645
GGACCGG200.006954741752.484931
CGCGTGT200.006954741752.484937
GCGCGTG305.8804476E-446.653276
CGTGCGA305.8804476E-446.6532710
CGAGAAT305.8804476E-446.6532714
GAGGCAC602.582765E-740.84967822
ATATTCG350.001255996939.98851850
CAGTATT459.200523E-538.87772814
GACAATT501.7050619E-435.0079934
CGTTTAT400.002413770435.0079933
TCTTCGC400.002415806335.00197639
CGTGTGG601.2158231E-534.9899529
GTACGCG400.002419882534.9899525
GTCGCCG751.4901088E-632.6572951
ATTAGAG450.004294806631.12356218
GACACAT450.004294806631.12356241
GCTGACC450.004294806631.12356224
Produced by FastQC (version 0.11.5)