FastQCFastQC Report
Wed 25 May 2016
SRR1780957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291139
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT245088.417972171368316No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG42411.4566925077025064No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC23140.7948093522338128No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT12390.4255699167751486RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7550.25932630118259664No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4130.14185663892504954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3750.12880445422976655No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT3600.12365227606057588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCT150.002223231170.0000142
CGTCCGC150.002223231170.0000137
AGGGGGG31900.060.3448370
CAACTCG601.8189894E-1258.33333636
CGTGCTA252.388062E-455.99999628
CCAGGAT200.006946849652.53
ACACCGG200.006946849652.526
GACGTGG200.006946849652.560
CAACAGG200.006946849652.524
CGAGATG200.006946849652.54
GATCTAC200.006946849652.51
ATCGGTT200.006946849652.525
TATCGGT200.006946849652.524
AACTCGT552.2773747E-950.9090937
CCGTCTT39200.048.30357447
GCCGTCT39650.048.28499646
AGTCTTC800.048.12548
CGAATGC800.048.12541
CGTCTTC39700.047.95969848
TCTTCTG38900.047.95629550
Produced by FastQC (version 0.11.5)