FastQCFastQC Report
Wed 25 May 2016
SRR1780956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208687
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5346425.619228797193884No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG3830.18352844211666275No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTAC150.002217881170.0354952
GATATTA207.902972E-570.01871
TGTGACG150.002224217969.98514638
GTGACGA150.002224217969.98514639
GCGCCTC308.3310915E-658.36290754
AATGGCG308.3310915E-658.36290750
TCGCCGT850.053.55655352
GCCGTAT850.053.55655354
GCTGCGG200.0069367752.51402317
GGTATAC200.0069367752.5140231
CCGGGGC200.00694335152.5014413
TCGGTGG1000.052.48885745
TGACGAT200.00694993752.48885340
TGCGCGG200.00694993752.4888535
CTGTGAC200.00694993752.48885337
TGGTCGC950.051.5803649
GTCGCCG900.050.5811951
CGGTGGT1000.048.98959746
ATATTCC305.8674905E-446.66794262
TCACGCC451.6815738E-646.6567643
Produced by FastQC (version 0.11.5)