FastQCFastQC Report
Wed 25 May 2016
SRR1780956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208687
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3326615.940619204837866No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG58572.806595523439409No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC22691.0872742432446678No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT15090.7230924782089924TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC12220.5855659432547308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6650.318659044406216No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5690.2726571372438149TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT4680.22425929741670542No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT3730.17873657678724597No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT3150.15094375787662864No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT2920.13992246761897004No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2780.13321385615778655RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2760.1322554830919032No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2250.1078169699118776No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT2200.1054210372471692No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT2140.10254591804951914RNA PCR Primer, Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAA150.002219178270.0251826
CCGTCAA207.9087404E-570.0083947
ATCGTAA150.002221289870.0083939
ATATCGA150.002221289870.0083937
CGTAATC207.9087404E-570.0083948
CTTAGTC150.002221289870.0083953
CGTCAAC252.8308295E-670.00838548
AGGGCGG207.918138E-569.99161570
CTTGTTG150.002223402769.9916113
AGAACCT150.002223402769.9916159
AGGGGGG40300.062.95771870
CGTCTAC453.8198777E-1062.22967548
AAGCCGT800.061.25734344
CCTTATA352.9665534E-759.9928051
AGTGCAA308.362114E-658.3263418
AAACTCG1050.056.6734636
AGCCGTC750.056.0067145
AATATCG1000.056.0067136
TACGAAT252.385075E-456.0067148
AACTCGT1000.056.0067137
Produced by FastQC (version 0.11.5)