FastQCFastQC Report
Wed 25 May 2016
SRR1780955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302557
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3678612.158370158350328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAT150.002220859370.0195121
AACCGAG150.002223773969.9963647
CTTCGCG200.00693945752.51463723
TCGAGCA200.00694399652.5059537
CGTGTGC200.00694853852.4972721
GGTTATC200.00694853852.4972721
GTCGCCG608.367351E-1152.4972751
GCCGTAT552.2791937E-950.90644554
CGGTGGT506.1836545E-848.9974646
TCGGTGG506.1836545E-848.9974645
TGTGCGC506.19184E-848.9893573
GTCCGGA305.869217E-446.67195533
TCGCCGT604.9221853E-946.66423852
ATGTACC305.874002E-446.66423848
GTGCGCG451.6841641E-646.6565324
ATCTCGG551.3071622E-744.55050342
CTCGGTG503.4735986E-641.9978244
CGTGCGA350.001255643439.9913110
AGATCTC708.664083E-735.00396740
CAACGTC501.706373E-435.00396734
Produced by FastQC (version 0.11.5)