FastQCFastQC Report
Wed 25 May 2016
SRR1780955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302557
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT214027.073708425189302TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG37211.2298509041271561TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC18400.6081498692808297RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT13700.45280723962757435TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7790.2574721457444383RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3820.12625720112243313TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3490.11535016542337476No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT3260.1077482920573644No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTCG150.002224003269.99454510
AGCGTCA352.9701914E-759.9953273
CTTTATA1400.059.9953272
GCAGCGT352.9701914E-759.9953271
AGGGGGG26650.058.57922770
AACTCGT308.360907E-658.3384337
CGACTTC252.3852321E-456.0141548
CGTCTTA452.6893758E-854.45820248
CGAATGC452.692832E-854.449241
ACGTAAT200.00694017152.51326847
CATTCGA200.00694925252.49591411
CGAATTT200.00694925252.49591434
GGCATAT200.00694925252.4959141
AAAGCGT352.0847567E-549.99610535
CCGTCTT33650.048.3777547
ATCGTAT800.048.12920839
GCCGTCT33900.048.12425246
CGTCTTC34050.048.1178848
TATGCCG35600.047.68675243
ATCTCGT35400.047.46177337
Produced by FastQC (version 0.11.5)