FastQCFastQC Report
Wed 25 May 2016
SRR1780954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793989
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597277.522396405995549No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG103911.3087083070420371No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12540.15793669685600178No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT11510.14496422494518188No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG9260.11662630086814806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC200.006947341452.50746539
GTTGCGT200.006955999452.4909321
GCCGTAT17450.047.92868454
TCGCCGT17400.047.8652952
CGGTGGT17650.047.58684546
TGGTCGC17800.047.3794549
CTCGGTG17750.047.12455744
TCGGTGG17950.046.98648545
GGCGTTA305.8833056E-446.6586071
GCTCTAC305.8833056E-446.6586071
TCTCGGT18100.046.6000343
GTCGCCG18000.046.46419551
GATCTCG17950.046.40743641
ATCTCGG17950.046.40451442
AGATCTC18250.046.2257540
CGTATCA18400.045.6500456
CGCCGTA18250.045.63595253
GTAGATC18650.045.4248738
GTATCAT18500.045.214157
TAGATCT19000.044.40105439
Produced by FastQC (version 0.11.5)