FastQCFastQC Report
Wed 25 May 2016
SRR1780954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793989
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT336544.238597763948871No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170872.1520449275745634No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT51890.6535355023810153No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG42570.5361535235374798No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC17530.22078391514240123No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT12770.1608334624283208RNA PCR Primer, Index 48 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT9980.12569443657279888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA207.927534E-569.99924530
AGGGGGG54950.059.93943470
CTTCGCG252.3907128E-455.99939714
CCGTCTT58550.054.33758547
CGTCTTC59200.054.33218448
GCCGTCT59000.054.16043546
TCGTATG60750.053.92534340
CGTATGC60150.053.82319341
AATCTCG60400.053.77425836
TATGCCG59650.053.74627743
CTTGAAA60650.053.6680157
ATCTCGT60100.053.63502537
CTCGTAT60500.053.4539739
TCTTCTG60650.053.43718350
GTCTTCT61000.053.18794649
ATGCCGT60500.053.1647244
TCTCGTA60850.053.14650738
CTGCTTG60700.053.10486254
TGCCGTC60650.053.09093545
GTATGCC60600.052.90371342