FastQCFastQC Report
Wed 25 May 2016
SRR1780953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588038
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5901410.035745989204779No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG106461.8104272172886786No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT11430.19437519343987975No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC11320.19250456603144694No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG10080.1714174934272955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCG200.006950089252.5005141
AGCGTAG200.006954766352.491582
TGTCGAC200.00695710652.48711810
GCCGTAT16200.049.47999654
TGGTCGC16550.049.27121449
CGGTGGT16550.049.2670246
TCGCCGT16450.049.15359552
TCGGTGG16700.049.0340545
GTCGCCG16650.048.7733951
CTCGGTG16600.048.48620244
GATCTCG16650.047.9283941
CGTATCA16850.047.7790156
AGATCTC16750.047.64225440
ATCTCGG16800.047.4964242
GTATCAT17000.047.34937757
TCTCGGT17000.047.1435143
GTAGATC17100.047.0764838
TAGATCT17150.046.9392339
GGTGGTC17450.046.72603247
CGCCGTA17250.046.6710953
Produced by FastQC (version 0.11.5)