FastQCFastQC Report
Wed 25 May 2016
SRR1780953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588038
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT324715.521922052656461No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174522.967835411997184No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT54660.9295317649539656No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG42380.7207017233580143No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC19660.33433213499807835No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT13330.2266860304946279No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT11520.19590570677405203TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6310.10730599042918995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAG150.00222483469.99625466
AGGGGGG55100.061.6119570
TGCCGTA252.3897004E-456.00176645
CGTCTAC653.6379788E-1253.85243648
AAGGGGG63950.053.03078569
GCCGTCT62500.052.53412646
TATGCCG63450.052.5154543
GAACGGT200.006949487652.501666
CCGTCTT62100.052.42157447
CGTCTTC62950.052.32540548
CGTATGC64450.052.29800841
TCGTATG65450.052.1941640
CTCGTAT65100.052.0984239
CTTGAAA65400.052.06908457
TCTCGTA65100.051.99088738
GTATGCC64200.051.9019542
ATCTCGT65300.051.88525437
GTATCTC65050.051.8112235
ATGCCGT64250.051.75260544
CACGAAT1150.051.73636632