FastQCFastQC Report
Wed 25 May 2016
SRR1780952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203816
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3920719.23646818699219No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG60792.9825921419319386No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC7420.36405385249440675No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT6520.31989637712446517No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG4610.22618440161714487No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG2770.13590689641637554No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT150.002225239569.976457
TAGACTC252.3874296E-455.994919
GTGTTGC252.390327E-455.981161
TCGGGAT252.390327E-455.981164
CCATCGG252.390327E-455.981161
CGGCTAG200.006926167852.5338930
GGCAGTT200.00693289952.52099238
AACGTCT200.006939634752.508123
CATATAG200.006953121652.4823343
GCCGTAT10500.049.9954454
TGGTCGC10700.049.71509649
TCGCCGT10650.049.61989252
ATCTCGG10650.049.61989242
GATCTCG10650.049.61989241
AGATCTC10700.049.40014640
CGTATCA10650.049.2791956
TCTCGGT10800.049.2547743
TAGATCT10850.049.0518639
GTAGATC10950.048.92366838
GTCGCCG10900.048.8028951
Produced by FastQC (version 0.11.5)