FastQCFastQC Report
Wed 25 May 2016
SRR1780952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203816
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2193010.759704831809083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100884.949562350355222No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT32361.5877065588570085No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG29681.456215409977627No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC11030.5411743925893944No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT8810.4322526200102053No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT8570.4204772932448876RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4120.20214310947128783No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2900.142285198414256No Hit
ATTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2790.13688817364681868No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2570.1260941241119441No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG150.002222333669.9995121
CGCGTGT150.002222333669.9995126
TTCGCGT150.002222333669.9995124
TAAACGG207.913276E-569.99950426
AGGGGGG35600.063.4124870
GCCGACT352.9638977E-759.9995846
GACAACG308.35583E-658.33292818
CGTATGA800.056.874641
AAACGGC252.3864387E-455.99960727
GTAAACG252.3864387E-455.99960725
AAGGGGG40300.055.9301369
TAAACAC6850.055.182093
CTTAAAC5600.054.3879621
CCGTCTT40300.054.10632347
GCCGTCT40700.054.0905346
CGTCTTC40900.054.08275248
ATCTCGT41500.053.6381837
TATGCCG41300.053.5589543
AACACAT7850.053.5028085
TGCCGTC41100.053.39378745