FastQCFastQC Report
Wed 25 May 2016
SRR1780951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329526
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4408813.379217421387082No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG85682.600098323045829No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT8750.2655329169777195No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC8530.25885666077942254No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7130.21637139406298747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCATG150.002222956470.0042532
TCCGAGA200.006937652452.5191351
GCCGTAT14600.046.28398554
TGGTCGC14700.046.18626849
TCGGTGG14700.046.1792645
TCGCCGT14750.046.05067452
CGGTGGT14700.045.9412246
CGTATCA14800.045.88813456
GTAGATC14700.045.7170638
CTCGGTG14700.045.70318244
GTCGCCG15100.045.6788951
CCGTATC14850.045.5047955
ATCTCGG14700.045.47204642
TCTCGGT14700.045.47204643
CGCCGTA14850.045.26901653
AGATCTC14850.045.248440
GATCTCG14800.045.1648141
TAGATCT14800.044.93516539
GGTGGTC15300.044.5970147
GTATCAT14950.044.47080657
Produced by FastQC (version 0.11.5)