FastQCFastQC Report
Wed 25 May 2016
SRR1780951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329526
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT253507.692867937583074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138064.189654230622166No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT37621.1416398099087781No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG33931.0296607854918882No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14330.4348670514617966No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT8280.2512700060086306No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT7300.221530319307126Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4640.14080831254589926No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGC150.002224639369.990922
TCGAATG650.064.62659540
AGGGGGG39900.062.1066170
TAAACAC7850.057.9542433
ATATCGT800.056.88486537
CGAATGC750.056.00971241
GGACCTA252.390062E-455.9927141
CTTAAAC6450.055.8842051
CCGTCTT42300.055.36421247
GCCGTCT42600.055.3030246
CGTCTTC42450.055.25104548
AAGGGGG44750.055.06264569
CTTGAAA43550.054.89211757
TATGCCG43350.054.7499843
ATCTCGT43150.054.6792437
CGTATGC43750.054.64948341
ATGCCGT43550.054.5789344
TCGTATG44450.054.57639740
TCTCGTA43900.054.5424938
CTCGTAT43850.054.52485339