FastQCFastQC Report
Wed 25 May 2016
SRR1780950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212557
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4795022.55865485493303No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG90004.2341583669321645No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC9390.4417638562832558No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT9000.4234158366932164No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG8000.3763696326161924No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG3330.15666385957649007No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTA150.002221721570.0054225
GTCGGAC150.002221721570.0054212
CGGGTTG200.00693569952.51641569
TCTCGTT200.00693569952.51641570
AGCGCTT200.00693569952.51641568
CAGGTAT200.006942158652.504061
TAAACCG200.006942158652.5040618
CGTCTAC200.006942158652.504061
CGCTAAA200.006942158652.5040622
GGTATTG200.006942158652.504061
ACGCTCT200.006942158652.5040620
GTATACC200.006942158652.504062
TGTAGCG200.006942158652.5040617
AAGGATC200.006942158652.5040623
GTCGAAA200.006948622452.49170729
CGTGAAA200.006948622452.49170734
TGTACCG200.006948622452.4917075
TCTCGGT14250.051.07964743
TCGGTGG14400.050.79058545
CTCGGTG14350.050.7236944