FastQCFastQC Report
Wed 25 May 2016
SRR1780950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212557
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2742312.901480544042304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140966.631632926697309No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT42742.0107547622520077No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG38831.8268041043108436No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC14240.6699379460568224No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT9650.453995869343282RNA PCR Primer, Index 42 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT9170.43141369138631047No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5460.2568722742605513No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3590.16889587263651634RNA PCR Primer, Index 32 (95% over 21bp)
ATTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3570.16795494855497584No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2360.11102904162177674No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2300.1082062693771553No Hit
CTAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2160.10161980080637195No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2130.10020841468406122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC455.456968E-1270.0028248
CCGACTT401.3460522E-1070.0028247
AGGGGGG40000.064.0676670
CTTAAAC5750.060.85771
TTAAACA6850.058.7614942
CGACTTC509.749783E-1056.0022648
TAAACAC7750.055.0989953
AAGGGGG46500.054.88609369
ATCTCGT47500.054.76246337
TCTCGTA48050.054.64565338
CTCGTAT48300.054.4352939
TATGCCG48300.054.42248543
GTATGCC48450.054.39847642
TCGTATG49000.054.37212840
CGTATGC48800.054.36694741
ATGCCGT48150.054.2285644
GCCGTCT48200.054.09969346
CGATCTC47500.054.0863935
GATCTCG47950.054.02948836
TGCCGTC48200.054.02707745
Produced by FastQC (version 0.11.5)