FastQCFastQC Report
Wed 25 May 2016
SRR1780949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254773
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5775722.669984653004832No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG121184.756390983345959No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT12960.5086881262928176No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC10330.4054589772071609No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7580.2975197528780522No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG4360.17113273384542319No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG150.002219574370.0265155
CGACGAA252.3853427E-456.0102122
GTACTCA200.006940850552.5095724
CATCGGT200.00694624352.499262
AAGACCG200.006951638552.4889565
TCGCCGT18500.051.85747552
CGCCGTA18500.051.85747553
GCCGTAT18450.051.80823554
CGTATCA18600.051.74658256
GTGGTCG18850.051.42154748
TCGGTGG18850.051.42154745
TGGTCGC18800.051.39234549
CCGTATC18600.051.39042355
CTCGGTG18800.051.37217744
TCTCGGT18800.051.37217743
GTCGCCG18800.051.216251
CGGTGGT18900.051.10036546
GTATCAT18900.051.10036557
GGTCGCC19000.050.6770850
GGTGGTC19250.050.53482447
Produced by FastQC (version 0.11.5)