FastQCFastQC Report
Wed 25 May 2016
SRR1780949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254773
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3347013.137184866528242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187077.342614798271402No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT52312.0532002998747902No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG45681.7929686426740667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC12040.47257754942635205No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT10170.39917887688255815RNA PCR Primer, Index 15 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT8400.329705267041641No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5610.22019601763138166No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4880.1915430599003819No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC4060.15935754573679314No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3760.14758235762816313No Hit
ATTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3630.14247977611442342No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2930.11500433719428668No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2730.10715421178853332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA150.002221699670.0096331
CGGCTAT150.002221699670.0096343
CCGACTT252.832332E-670.0096247
ACGACTA207.911406E-570.0096247
GGAACGT150.002223430469.9958816
GCGCTCA150.002223430469.9958833
AATATCG1200.064.1754936
AGGGGGG50800.063.17541570
CGACTTC453.8380676E-1062.23077448
ATCGTAT1200.061.25842339
GACGACT401.0615622E-861.2584246
CTTAAAC8200.061.032991
AACACAT11000.060.132825
TAAGCCG352.9638977E-760.00824443
TAAACAC10150.058.9620443
ATATCGT1250.058.80808337
TTAAACA9800.057.853742
TAATATC1300.056.5351335
AAACACA12450.056.5026974
ACAACGT252.3858724E-456.00769844
Produced by FastQC (version 0.11.5)