FastQCFastQC Report
Wed 25 May 2016
SRR1780948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471413
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5235311.105548637818643No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG80791.713783879528142No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC9010.1911275251212843No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT8880.18836985827713704No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7560.16036893339810315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC252.390498E-455.9956743
CGGTATG200.00695162952.49594543
GCCGTAT12650.047.0319454
GATCTCG12900.046.39668741
ATCTCGG12950.046.2175542
GTAGATC13200.046.1376938
TCGCCGT12850.046.0275752
TCGGTGG13200.045.86766445
GTCGCCG13100.045.6834951
CTCGGTG13100.045.6834944
CGCCGTA12950.045.67214253
CGGTGGT13300.045.52279746
AGATCTC13250.045.43527240
TAGATCT13250.045.43527239
TGGTCGC13100.045.41633649
CCGTATC13600.043.74661655
CGTATCA13700.043.6827656
GTATCAT13750.043.5239157
TCTCGGT14100.043.1881543
GGTGGTC14200.042.6375547