FastQCFastQC Report
Wed 25 May 2016
SRR1780948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471413
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT296456.288541045749693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134242.847609208910234No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT42300.8973023654417677No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG39130.8300577200883301No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC15150.321374251452548No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT12070.2560387600681356No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT10630.22549229656373498Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5420.11497349457906336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC207.92419E-569.9992545
AACGGAT150.002224181569.9992525
CGTATGA750.065.3326441
AGGGGGG48050.060.82141570
CAGTAGT308.372983E-658.3327141
GTCTTCA800.056.87439349
TATGCCG53250.054.29050443
CGTCTTC51900.054.2865148
ATCTCGT54000.054.24942437
CCGTCTT51450.054.2171147
TCTCGTA54250.054.1929738
GATCTCG54450.054.18675636
CTCGTAT54000.054.1846139
TCGTATG54650.053.98845340
AGATCTC54750.053.88984335
CTTGAAA53400.053.8758357
GCCGTCT52300.053.87132346
GTATGCC53700.053.83555242
CGTATGC54300.053.8207941
AAGATCT55250.053.5921934