FastQCFastQC Report
Wed 25 May 2016
SRR1780947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470384
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5774712.276565529439777No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG93861.9953909996938672No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC10250.21790707166910436No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT9720.20663968162182386No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7400.1573182761318412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA150.002223173470.0072356
TCACGCG200.006952501452.4942660
ATGCGTG200.006952501452.4942658
CTTTATA850.049.4116132
GCCGTAT14000.049.24984754
GTAGATC14550.048.36579538
TCGCCGT14050.048.32725552
CTCGGTG14550.047.8641844
CGGTGGT14550.047.8641846
TCGGTGG14550.047.8641845
AGATCTC14650.047.77634440
GATCTCG14650.047.54251541
TGGTCGC14500.047.5414749
TAGATCT14750.047.4675839
GTCGCCG14500.047.310251
TCTCGGT14800.047.29212643
ATCTCGG14800.047.05566442
CGTATCA14750.046.7505956
TAACGGT305.8666937E-446.68141636
AACGGTA305.8666937E-446.68141637
Produced by FastQC (version 0.11.5)