FastQCFastQC Report
Wed 25 May 2016
SRR1780947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470384
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT314036.67603489914623No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153143.255637946868941No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT44630.9487992788870369No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG40830.8680142181706861No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC15160.32228987380523144No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT11260.23937889043845031No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT8550.18176638661178954RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6080.12925609714616143No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGA207.918569E-570.00925444
TCGCACA150.002222919670.0092531
GACGTAT150.002222919670.0092546
AGGGGGG49500.061.5167470
ACGTATG308.360601E-658.34724440
AAGCCGT900.058.34103844
CCGTCTT48950.056.1361247
TGACGAC252.3880202E-456.007445
GCCGTCT49150.055.97891246
CGTCTTC48450.055.70395348
TATGCCG53400.055.58786843
CGTATGC52750.055.27744741
ATCTCGT54000.055.23539437
TCTTCTG46650.055.2152850
TGCCGTC52350.055.16488345
TCTCGTA54200.055.09616538
GTATGCC53950.055.0211742
ATGCCGT53700.055.0165844
TCGTATG53900.054.9481740
CTCGTAT53400.054.93819439