FastQCFastQC Report
Wed 25 May 2016
SRR1780946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512216
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5933911.584761116404017No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG102441.9999375263560686No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT12190.23798553735142985No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG10870.21221515922969997No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAGA150.002224407469.9983417
CGTGCGA150.002225268969.9915110
TGACGGC150.002225268969.9915147
CTGGTAT150.002225268969.9915113
GGTTCGC150.002225268969.991511
TTACTTG200.006950499552.49875326
GCGCAAC200.006950499552.49875327
ATACGTG200.006950499552.49875320
GATCTAC200.006953183652.493631
ACTGGTA200.006953183652.4936312
GTCGCCG15400.049.5394451
GCCGTAT15350.049.4728254
TCGCCGT15450.049.15261552
TGGTCGC15550.049.06157349
GATCTCG15650.048.97647541
CTCGGTG15600.048.90432444
TCGGTGG15750.048.66076345
GTAGATC15950.048.50361638
ATCTCGG15750.048.4385742
AGATCTC15950.048.27471540
Produced by FastQC (version 0.11.5)