FastQCFastQC Report
Wed 25 May 2016
SRR1780946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512216
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT343856.7129882705733515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159583.115482530807316No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT51451.0044590563356084No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG42470.8291423930529307No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC19380.37835600605994346No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT11520.22490511815327907Illumina PCR Primer Index 10 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT9390.18332109891139675No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6350.12397113717650367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT252.8347476E-670.01659439
AGGGGGG53100.060.8524770
TAACTCG700.060.01423336
ACGTATG308.361727E-658.34716840
GCCGTAT553.6379788E-1157.2695346
GTCTTCA1350.057.0338649
CCGGTTG252.3869833E-456.0132868
AAGGGGG59750.053.845469
CCGTCTT56100.053.8383547
TATGCCG58200.053.8200243
GCCGTCT56500.053.76690746
CGTCTTC57050.053.73933448
CGTATGC59000.053.62412641
ATGCCGT58600.053.57209444
AACTCGT850.053.54210337
TGCCGTC58000.053.46253245
TCGTATG60250.053.39855240
TCTTCTG56900.053.3889450
CTCGTAT60100.053.35707539
GTATGCC58750.053.31617442