FastQCFastQC Report
Wed 25 May 2016
SRR1780945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180800
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5173728.61559734513274No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG103055.6996681415929205No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC11660.6449115044247787No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT10430.5768805309734514No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7670.4242256637168142No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG3650.20188053097345135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA150.002215286570.0523329
AGCGTGG150.002215286570.0523327
GTTCGCA150.002215286570.0523335
CTATAAG150.002222595369.99419447
GCGCTAT150.002222595369.99419419
GGGATCC150.002225035669.9748463
CTTTATA453.8380676E-1062.199852
TGCCGGC308.355781E-658.32849519
CACGCTA252.3800894E-456.0263523
TACCACG252.3833477E-456.01084520
GTACAAC252.3866096E-455.99535443
GTTCTAC200.00692211552.53924635
GACGTCC200.00692211552.53924631
TGCGGCA200.00692211552.53924635
AGAGTAC200.00692211552.53924628
GTCCGGG200.00692211552.53924634
ACGTCCG200.00692211552.53924632
CGTCCGG200.00692211552.53924633
GGATACC200.006929752.52470426
GCAAATG200.006944887352.49564743