FastQCFastQC Report
Wed 25 May 2016
SRR1780945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180800
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3014016.670353982300885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164859.117809734513274No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT43132.385508849557522No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG40632.247234513274336No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC16550.9153761061946902No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT9660.5342920353982301RNA PCR Primer, Index 24 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT9630.5326327433628318No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC5000.27654867256637167No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4210.23285398230088494No Hit
ATTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3220.17809734513274336No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2920.16150442477876106RNA PCR Primer, Index 24 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2860.1581858407079646No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2000.11061946902654868No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1870.10342920353982302No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGAC150.002221864670.000013
ATCGTAA150.002221864670.0000139
AGCGGTT150.002221864670.0000112
ATGACGA150.002221864670.0000144
TCCGTAC150.002221864670.0000140
CTCTGGT150.002221864670.0000132
CATTTGC150.002221864670.0000119
CTTAAAC6450.066.744191
ACGAGAT900.066.1111127
TTAAACA7400.064.3243262
TAGCAAC600.064.1666733
AGGGGGG46250.063.71891870
CGTCTTA501.4551915E-1162.99999648
TAAACAC8000.062.5624963
TTAATAC453.8198777E-1062.2222183
AATCTCG401.0604708E-861.2536
GCCGTAT401.0604708E-861.2546
TGCCGTA401.0604708E-861.2545
AAACACA9700.060.979384
AACACAT9350.060.6417165
Produced by FastQC (version 0.11.5)