FastQCFastQC Report
Wed 25 May 2016
SRR1780944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164366
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5834035.49395860457759No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG114756.981370843118406No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT12700.7726658798048258No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC11330.6893153085187934No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG9400.5718944307216821No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG3760.22875777228867286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT150.002217251370.03380622
TTTCCGA150.002217251370.03380629
CCGACGG150.002217251370.03380632
AGTACGT150.002217251370.03380638
GTACGTG150.002217251370.03380639
TACGTGT150.002217251370.03380640
CGACGTA150.002222613769.9911753
TCGACGT150.002222613769.9911752
TCATCTG150.002225298769.969888
TCAAATG352.95031E-760.02897340
TCGGCTT252.3900683E-455.97590614
GCCGAGT252.3900683E-455.97590658
CGGCTTA252.3900683E-455.97590615
GGCGCTG252.3900683E-455.97590614
AAAAAGG9500.054.1675570
AAGTAAT200.006928244652.5253521
TCCGACG200.006928244652.5253531
TTCCGAC200.006928244652.5253530
GGGAGCG200.006928244652.5253520
ACGTGTC200.006928244652.5253541