FastQCFastQC Report
Wed 25 May 2016
SRR1780944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164366
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3326220.236545270919777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1802110.963946314931313No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT59243.604151710207707No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG42092.5607485733059145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC13630.829246924546439No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT12140.7385955732937469No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT11780.7166932333937676TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6340.3857245415718579No Hit
ATTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4890.2975067836413857No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4300.2616112821386418TruSeq Adapter, Index 12 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3160.19225387245537398No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2480.15088278597763527No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2440.14844919265541537No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2340.14236520934986555No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2340.14236520934986555RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2050.12472165776377109No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT1820.11072849616100652No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT1760.10707810617767664No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1730.10525291118601171RNA PCR Primer, Index 12 (95% over 22bp)
ATACCCATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT1690.10281931786379178TruSeq Adapter, Index 12 (95% over 23bp)
CATAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1670.10160252120268182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG401.3460522E-1070.01491536
GTCTGCC207.900102E-570.01491542
CTGCCGT207.900102E-570.01491544
TGCCGTA401.3460522E-1070.01491545
CAAAAGC150.002219627170.0149127
TGACGTT150.002219627170.0149129
ATGTTCT150.002219627170.0149136
TACGAGA150.002222308469.9936155
TCTAATG150.002222308469.9936150
CACAGAT150.002222308469.9936116
GATTCCG150.002222308469.9936113
CTGATAG252.8317008E-669.9936154
TTCTGAC150.002222308469.9936111
TTAAACA9450.065.919912
CTTAAAC8550.065.900411
TCGAATG850.065.89638540
TAAACAC10050.065.814893
AGGGGGG50550.064.6627470
CGTCTAC650.064.6291448
AAACACA11700.062.515664
Produced by FastQC (version 0.11.5)