FastQCFastQC Report
Wed 25 May 2016
SRR1780943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527559
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6064211.494828066623827No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG103791.967362892112541No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT10720.20320002122985298No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC10270.1946701695924058No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG9480.17969554116222072No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACGT200.006956105652.4882741
CGCGTGT200.006958713352.4832957
CTGGACG352.0898564E-549.9840934
GCCGTAT16400.049.08370254
CTCGGTG16550.049.06643744
CGGTGGT16800.048.7344946
TCTCGGT16850.048.60830343
TCGGTGG16900.048.459945
TCGCCGT16550.048.4273652
AGATCTC16700.048.21109840
GATCTCG16750.048.0626341
TGGTCGC16950.047.89943749
GTCGCCG16850.047.7728651
ATCTCGG17050.047.4222542
CGTATCA17150.047.3363656
CGCCGTA17050.047.2124853
TAGATCT17150.046.94608739
ACGTGTA305.88297E-446.6562462
GTAGATC17300.046.5390438
GTGGTCG17750.046.12616348
Produced by FastQC (version 0.11.5)