FastQCFastQC Report
Wed 25 May 2016
SRR1780943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527559
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT345816.554906654990248TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167813.18087645173336No Hit
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT49280.9341135304297717RNA PCR Primer, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG41060.7783015738524033TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC15380.2915313737420838RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT11830.22424032193555601TruSeq Adapter, Index 5 (96% over 26bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT9370.1776104663175114No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6240.11828060937260099RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG52900.060.6772470
CGAATGC352.972738E-760.00040441
GTACTAG252.3897676E-456.0003781
TATGCCG58800.054.7027543
CCGTCTT56200.054.68008847
CGTCTTC56700.054.5682748
GCCGTCT56550.054.5273446
CGTATGC59700.054.40572741
TCGTATG60600.054.35885640
ATCTCGT59800.054.3250537
ATGCCGT59000.054.2800344
TGCCGTC58200.054.2443545
GATCTCG60350.054.17799436
CTCGTAT60100.054.17037239
GTCTTCT56600.054.16997549
TCTCGTA60200.054.08038738
GTATGCC59550.054.0725742
CTTGAAA58600.053.75972457
TCTTCTG56950.053.70905350
TGATCTC59950.053.54171435