FastQCFastQC Report
Wed 25 May 2016
SRR1780942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436648
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4405310.088904563859218No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG60851.3935710228834208No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT6930.15870907458639452No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5600.12824975724153095No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4540.1039739103350983No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG150.002224092869.99908454
ATGTCGT200.00694322952.5113428
CCGAGAC200.00694952352.49931354
CGAGACA200.00694952352.49931355
AACTCCG305.879072E-446.6607137
GCCGTAT10600.045.56544554
ATCTCGG10850.045.48327342
GTAGATC10950.045.3927338
AGATCTC10800.045.36977840
TCTCGGT11000.045.18122543
TGGTCGC10900.044.9535449
CTCGGTG11000.044.8579144
TCGCCGT10850.044.51554552
CGGTGGT11100.044.45378546
TAGATCT11150.044.2595139
TCGGTGG11300.043.9766945
GATCTCG11250.043.55498541
CGTATCA11200.043.43195756
GTCGCCG11200.043.12443551
CGCCGTA11200.043.12443553