FastQCFastQC Report
Wed 25 May 2016
SRR1780942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436648
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT239625.48771550539565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96712.21482750407651No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG40850.935536175592239No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT36910.8453033106758762No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT14200.3252047415767392RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC13790.3158150272072699No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6690.15321265641890033No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4420.1012257012513512No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTC207.924146E-569.998176
TACGGCA150.002224208669.998172
AGGGGGG39000.059.49844470
ATCGTAT1300.053.84474639
GCGAATC200.00694988352.49862715
TTCGTAA407.4749187E-752.49862730
AAGGGGG47050.049.0210369
ATTCGTC506.1852916E-849.00432629
CGTCTTC47250.048.9616848
CCGTCTT46400.048.802647
CTTGAAA48400.048.74397757
GCCGTCT47250.048.73946846
TATGCCG47150.048.62014843
CGTATGC48050.048.58353441
TCGTATG49350.048.43844640
GTCTTCT47700.048.2796649
CTTTATA1450.048.2745972
CTCGTAT48150.048.19188739
TCTTCTG47850.048.1283150
ATCTCGT48450.048.11019537