FastQCFastQC Report
Wed 25 May 2016
SRR1780941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594441
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346895.83556652384341No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG42610.7168078917840459No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG150.002224151570.0017742
TGTGCGC252.8402974E-669.995883
TGCGCGT252.3907902E-455.9967045
GGTGTCG200.006947388452.5057441
GTACTAG200.00695201352.496911
GTGTGCG352.086976E-549.9970552
GTGCGCG352.086976E-549.9970554
GCCGTAT8200.049.08247454
TCGCCGT8300.048.4911252
TGGTCGC8550.047.89191449
GTCGCCG8600.047.61347651
CTCGGTG8600.047.21049544
CGGTGGT8800.046.93300646
TCTCGGT8750.046.80117843
CGCAGCT305.878805E-446.66391854
GATCTCG8700.046.2694341
TCGGTGG8800.046.1375345
CGCCGTA8900.045.6152953
GTAGATC9000.045.5011538
AGATCTC9000.045.1122540
Produced by FastQC (version 0.11.5)