FastQCFastQC Report
Wed 25 May 2016
SRR1780941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594441
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT161692.7200344525360802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72321.2166051803290823No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT46300.7788830178268323No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG28620.4814607336977093No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT10400.17495428478183706TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC8970.15089807062433447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGA150.002224448669.9994145
ATCGGTA150.002224448669.9994126
CGTCTAC453.8562575E-1062.221748
CGAATCT800.061.2494934
GCCGACA308.375551E-658.33284446
AGGGGGG32700.056.2992270
GGGTCTA252.3882076E-456.0089531
TAACTCG700.054.99953536
TGCCGAC700.054.99953545
GATCGGT200.006950624352.4995625
CTCTCGT200.006950624352.4995637
ACGAATC950.051.5785133
GCCGTCT33400.051.34687446
CCGTCTT33000.051.332947
CGTCTTC33950.051.23668348
CGTATGC36650.051.09097741
CACGAAT1100.050.9086632
AATCTCG1100.050.9086636
CGACTTC552.2846507E-950.9086648
TATGCCG35350.050.8906643
Produced by FastQC (version 0.11.5)